abstract：:Agonist induced increases in intracellular free calcium, [Ca2+]i, were measured in single Fura-2 loaded bovine aortic endothelial (BAE) cells by dual wavelength microspectrofluorimetry. Low doses of ATP (less than 10 microM) induced complex changes in [Ca2+]i. These changes usually consisted of a large initial transie...

journal_title：Cell calcium

authors： Lynch M,Gillespie JI,Greenwell JR,Johnson C

更新日期：1992-04-01 00:00:00

abstract：:A potential role of arachidonic acid in the modulation of insulin secretion was investigated by measuring its effects on calmodulin-dependent protein kinase and protein kinase C in islet subcellular fractions. The results were interpreted in the light of arachidonic acid effects on insulin secretion from intact islets...

journal_title：Cell calcium

authors： Landt M,Easom RA,Colca JR,Wolf BA,Turk J,Mills LA,McDaniel ML

更新日期：1992-03-01 00:00:00

abstract：:The effects of acute (3 h) and chronic (30 h) in vivo infusions of Escherichia coli endotoxin on the Ca2+ homeostasis of rat spleen cells was investigated. Conditions were established for obtaining reliable estimates of [Ca2+]i in these cells using the newly-developed Ca2+ indicator Fluo-3. The resting [Ca2+]i of sple...

journal_title：Cell calcium

authors： Hagar AF,Spitzer JA

更新日期：1992-02-01 00:00:00

abstract：:Ca2+ pumps are essential for removing cytosolic Ca2+ either across the plasma membrane (PM) or into internal organelles such as the sarcoplasmic reticulum (SR). Four genes (PMCA1, PMCA2, PMCA3 and PMCA4) have been reported to encode the PM Ca2+ pumps and three (SERCA1, SERCA2 and SERCA3) to encode the SR Ca2+ pumps. T...

journal_title：Cell calcium

authors： Grover AK,Khan I

更新日期：1992-01-01 00:00:00

abstract：:Studies were conducted using normal and human papillomavirus Type 18 (HPV-18) immortalized human keratinocytes to assess possible alterations in the differentiation process as a consequence of increased intracellular calcium concentration. Normal keratinocytes exposed to increased extracellular calcium or the phorbol ...

journal_title：Cell calcium

authors： Garrett LR,Coder DM,McDougall JK

更新日期：1991-05-01 00:00:00

abstract：:A preparation of sea urchin eggs permeabilized with digitonin (40 microM for 2.5 min) was used to study the kinetic characteristics of the two cellular compartments suspected to play a key role in cellular calcium transfer during fertilization: an ATP-dependent Ca2+ pool (Km = 0.47 microM; Vm = 0.48 nmol/min.mg protei...

journal_title：Cell calcium

authors： Girard JP,Gillot I,De Renzis G,Payan P

更新日期：1991-04-01 00:00:00

abstract：:Receptor-activated cytoplasmic calcium (Ca2+) oscillations have been investigated in single pancreatic acinar cells by microfluorimetry (Fura-2 as indicator). At submaximal concentrations of the agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8), both give rise to oscillatory changes in the cytosolic...

journal_title：Cell calcium

authors： Yule DI,Lawrie AM,Gallacher DV

更新日期：1991-02-01 00:00:00

abstract：:Hepatocytes stimulated with calcium-mobilising agonists generate free Ca transients whose frequency is modulated by hormone concentration. Importantly, the time-course of individual free Ca transients is independent of agonist dose but does change with agonist species. A receptor-controlled model in which protein kina...

journal_title：Cell calcium

authors： Cobbold PH,Sanchez-Bueno A,Dixon CJ

更新日期：1991-02-01 00:00:00

abstract：:To determine intracellular Ca2+ concentrations more accurately, we examined Kd of Fura-2 for Ca2+ in conditions which were systemically changed. In a solution comprising of 150 mM KCI, 20 mM MOPS-KOH, pH 6.94, 60-100 microM EGTA and 1 microM Fura-2, Kd at 20 degrees C was 0.266 +/- 0.016 microM (mean +/- SEM) (21 dete...

journal_title：Cell calcium

authors： Uto A,Arai H,Ogawa Y

更新日期：1991-01-01 00:00:00

abstract：:Using single-cell ratio imaging of Fura-2-loaded neutrophils, we demonstrate that the heterogeneity and asynchrony of the oxidase response originates from variability in the timing and magnitude of the cytosolic free Ca2+ signal. The Ca2+ signals from individual cells could be classified into four types: (a) type 1, a...

journal_title：Cell calcium

authors： Hallett MB,Davies EV,Campbell AK

更新日期：1990-11-01 00:00:00

abstract：:Isolated avian growth plate chondrocytes convert the acetoxymethyl ester (AM) form of Fura-2 quickly and efficiently to the Ca2(+)-sensitive pentacarboxylic acid (FA) form. Control experiments indicate that the Kd for intracellular Fura-2/FA is very close to that of extracellular Fura-2/FA at the same ionic strength a...

journal_title：Cell calcium

authors： Gunter TE,Zuscik MJ,Puzas JE,Gunter KK,Rosier RN

更新日期：1990-08-01 00:00:00

abstract：:Glucose induces large amplitude oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i) in pancreatic beta-cells. The effects of temperature on these oscillations were examined by monitoring [Ca2+]i continuously in single beta-cells from ob/ob-mice using dual wavelength microfluorometry. The oscillations of [Ca2+...

journal_title：Cell calcium

authors： Hellman B,Gylfe E,Grapengiesser E,Panten U,Schwanstecher C,Heipel C

更新日期：1990-06-01 00:00:00

abstract：:Using the fluorescent Ca2+ probe Quin-2 it has been reported that cholera toxin (CT) and its B subunit (B-CT) increase cytosolic free Ca2+ concentration ([Ca2+]i) in entherocytes, thymocytes and fibroblasts. In this work we show, however, that the fluorescence increases of Quin-2-loaded cells (rat thymocytes, mouse sp...

journal_title：Cell calcium

authors： Astashkin EI,Surin AM,Mikhna MG,Nikolaeva IS,Lazarev AV,Gukovskaya AS

更新日期：1990-06-01 00:00:00

abstract：:When Xenopus oocytes injected with rat brain poly(A)+RNA were voltage-clamped in a recording solution containing Ca2+, a depolarization pulse induced a transient current, ICl(Ca), which reflects calmodulin-mediated opening of endogenous Cl- channels in response to a Ca2+ influx through Ca2+ channels of brain origin. I...

journal_title：Cell calcium

authors： Kaneko S,Doi E,Watanabe H,Nomura Y

更新日期：1990-04-01 00:00:00

abstract：:N,N'-dicyclohexylcarbodiimide (DCCD) and 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide (CMCD) inhibited calmodulin-dependent Ca2(+)+Mg2(+)-ATPase activity in erythrocyte ghost membranes. The extent of the inhibition caused by carbodiimides strongly depended on their hydrophobicity. Hydrophobic DCCD was a more potent...

journal_title：Cell calcium

authors： Famulski KS,Pikula S,Wrzosek A,Wojtczak AB

更新日期：1990-04-01 00:00:00

abstract：:The measurement of intracellular calcium ion concentrations [( Ca2+]i) in single living cells using quantitative fluorescence microscopy draws from a diverse set of disciplines, including cellular biology, optical physics, statistics and computer science. Over the last few years, we have devised and built a number of ...

journal_title：Cell calcium

authors： Moore ED,Becker PL,Fogarty KE,Williams DA,Fay FS

更新日期：1990-02-01 00:00:00

abstract：:We have recently shown that S-100b protein interacts with the polar surface of cardiolipin vesicles [6]. This interaction produces changes in the secondary structure of S-100b as well as changes in the structural organization of cardiolipin vesicles. We report here on the effects of S-100b on cardiolipin vesicles as i...

journal_title：Cell calcium

authors： Zolese G,Curatola G,Amati S,Giambanco I,Donato R

更新日期：1990-01-01 00:00:00

abstract：:We have investigated the effects of glucose on cytosolic free calcium concentration in the insulin-secreting cell line HIT-T15. Addition of glucose (10 mM) caused a 20-75% increase in cytosolic [Ca2+] within 5 minutes compared to controls in the absence of glucose. A maximal increase in cytosolic [Ca2+] was obtained w...

journal_title：Cell calcium

authors： Meats JE,Best L,Lynch AM,Tomlinson S

更新日期：1989-11-01 00:00:00

abstract：:Human neutrophils, preloaded with the fluorescent probe, Fura-2, were exposed to Ca2+-releasing agents. The monitored traces of fluorescence were transformed by computer to cytosolic Ca2+ concentration ([ Ca2+]i). Due to quenching of Fura-2, the addition of Mn2+ enabled us to compute the cytosolic concentration of tot...

journal_title：Cell calcium

authors： Foder B,Scharff O,Thastrup O

更新日期：1989-10-01 00:00:00

abstract：:Ca2+ signals are known to mediate an array of cellular functions including secretion, contraction, and conductivity changes. In spite of the obvious role of Ca2+ in signalling, the control of Ca2+ within cells is known to be a complex phenomenon involving a number of distinct active and passive transport systems funct...

journal_title：Cell calcium

authors： Gill DL,Ghosh TK,Mullaney JM

更新日期：1989-07-01 00:00:00

abstract：:An influx of Ca2+ into red blood cells of the bivalve mollusc Noetia ponderosa occurs immediately following a hypo-osmotic stress. The volume recovery response to the stress is dependent upon [Ca2+]o and is inhibited by phenothiazines. The action of these drugs is on the amino acid regulation portion of the recovery r...

journal_title：Cell calcium

authors： Pierce SK,Politis AD,Cronkite DH,Rowland LM,Smith LH Jr

更新日期：1989-04-01 00:00:00

abstract：:Rabbit gall-bladder epithelial cells were isolated by a combination of Ca2+ omission, enzymatic treatment, and mechanical detachment and had a viability of 96-98% and well preserved morphology. Measurements of cytosolic free Ca2+ concentration ([Ca2+]i) in these cells with the Ca2+-fluorescent indicator fura-2 demonst...

journal_title：Cell calcium

authors： Bouchelouche PN,Hainau B,Frederiksen O

更新日期：1989-01-01 00:00:00

abstract：:The annexins are a family of calcium-binding proteins. Data from protein and cDNA sequencing have shown that at least five distinct but closely related mammalian annexins exist each of which possesses four or eight homologous internal repeats which may be calcium-and phospholipid-binding domains. The proteins are pres...

journal_title：Cell calcium

authors： Burgoyne RD,Geisow MJ

更新日期：1989-01-01 00:00:00

abstract：:Calcium dependence of bleb formation and cell death was evaluated in rat hepatocytes following ATP depletion by metabolic inhibition with KCN and iodoacetate ('chemical hypoxia'). Cytosolic free Ca2+ was measured in single cells by ratio imaging of Fura-2 fluorescence using multiparameter digitized video microscopy. C...

journal_title：Cell calcium

authors： Nieminen AL,Gores GJ,Wray BE,Tanaka Y,Herman B,Lemasters JJ

更新日期：1988-12-01 00:00:00

abstract：:The highly differentiated structure of the neuron poses special problems for the intracellular movement of molecules throughout the cell. Molecular transport distances from the synthesizing neuron cell body along the axon (which has no substantial synthetic capabilities) to the axon terminal are very great. The transp...

journal_title：Cell calcium

authors： Breuer AC,Atkinson MB

更新日期：1988-12-01 00:00:00

abstract：:Under certain conditions of Ca2+ loading, cardiac myocytes, both isolated and in intact tissue, exhibit spontaneous, oscillatory Ca2+ transients due to Ca2+ release from the sarcoplasmic reticulum. These transients are not triggered by depolarization of the sarcolemma, though they themselves can generate depolarizing ...

journal_title：Cell calcium

authors： Stern MD,Capogrossi MC,Lakatta EG

更新日期：1988-12-01 00:00:00

abstract：:The present study was undertaken to determine in fetal rat osteoblasts whether and how the intracellular messengers calcium and cAMP are involved in stimulation of ornithine decarboxylase (ODC) activity. For that purpose we used different drugs affecting [Ca2+]i and cAMP concentration. A23187 stimulates ODC activity i...

journal_title：Cell calcium

authors： van Leeuwen JP,Bos MP,Herrmann-Erlee MP

更新日期：1988-08-01 00:00:00

abstract：:The highly selective fluorescent Ca2+ indicator 'quin 2' has been loaded into ram and boar spermatozoa as the acetoxymethyl ester, 'quin 2/AM', which is hydrolysed and trapped in the cytoplasm. Loadings of several mM were not toxic to spermatozoa as judged by motility. Fluorescence measurements (mean +/- S.E.M.) indic...

journal_title：Cell calcium

authors： Simpson AM,White IG

更新日期：1988-02-01 00:00:00

abstract：:Removal of the epithelium from preparations of guinea-pig airways in vitro increases the responsiveness of the smooth muscle of normal and ovalbumin-sensitized animals to a number of contractile agents. To determine if epithelium removal results in an increase in Ca2+ entry into the smooth muscle, the effect of removi...

journal_title：Cell calcium

authors： Raeburn D,Hay DW,Fedan JS

更新日期：1987-12-01 00:00:00

abstract：:It has been reported that leukotriene B4 can translocate calcium across model membranes (Serhan et. al., (1982) J. Biol. Chem., 257: 4746). Such ionophoretic behavior could account for its biological effects. We have examined the effect of chromatographically pure leukotriene B4 on Ca2+ permeability when added exogeno...

journal_title：Cell calcium

authors： Holmes RP,Yoss NL,Marshall LA

更新日期：1987-12-01 00:00:00

abstract：:To determine the effects of exposure to reserpine on subcellular Ca2+ transporting systems, active Ca2+ uptake was measured with and without ruthenium red in submandibular gland vesicles obtained from rats after chronic treatment with reserpine. The properties of ruthenium red-sensitive Ca2+ uptake were similar to tho...

journal_title：Cell calcium

authors： Hurley TW,Martinez JR

更新日期：1987-10-01 00:00:00

abstract：:The effect of Ca2+ ion concentration on the 25 hydroxylation of tritiated cholecalciferol (3HD3) was investigated using homogenates of ovine liver from vitamin D replete sheep. A significant decrease in the production of 25 hydroxycholecalciferol (25OHD3) was observed when the concentration of Ca2+ in the homogenate w...

journal_title：Cell calcium

authors： Corlett SC,Chaudhary MS,Tomlinson S,Care AD

更新日期：1987-08-01 00:00:00

abstract：:The Ca2+-sensitive dye quin-2 was used to measure the cytosolic free Ca2+ (Cai2+) in suspensions of ventricular myocytes isolated from cat and rat ventricles. Following an isolation procedure that was similar for both species, the cells were loaded with quin-2 AM (25 microM) for 30 min at 37 degrees C. After two washe...

journal_title：Cell calcium

authors： duBell WH,Houser SR

更新日期：1987-08-01 00:00:00

abstract：:Disturbances in intracellular Ca2+ are known to be important in cell injury caused by a wide range of toxic factors. The complement system is a major effector of immune damage in vivo, and is known to be involved in the pathogenesis of many immune diseases. We present here evidence that the potentially lethal membrane...

journal_title：Cell calcium

authors： Morgan BP,Luzio JP,Campbell AK

更新日期：1986-12-01 00:00:00

abstract：:Indo-1 is a new fluorescent indicator of the intracellular free calcium concentration Cai++. Indo-1 may be used in a similar manner as its predecessor quin2 but offers the principal advantage that the Ca++ saturated form of the Ca++ chelator has a emission maximum different in wavelength from that of free indo-1 (400 ...

journal_title：Cell calcium

authors： Lückhoff A

更新日期：1986-08-01 00:00:00

abstract：:The effects of the thyroid status on the Ca++-transporting capabilities of rat slow skeletal muscle (m.soleus) were studied. The oxalate supported Ca++-uptake activity and Ca++-loading capacity of muscle homogenates from hyperthyroid rats showed an approximate 4.2 and 2.5 fold increase, respectively, as compared to va...

journal_title：Cell calcium

authors： Simonides WS,van Hardeveld C

更新日期：1986-06-01 00:00:00

abstract：:Ca2+ efflux from dog red blood cells loaded with Ca2+ using the A23187 ionophore could be separated into two main components: (1) Mg- and ATP-dependent (active transport) and (2) dependent on external Na (K1/2 around 15 mM); at 80 microM internal free Ca the relative magnitudes of these fluxes were 70% and 30% respect...

journal_title：Cell calcium

authors： Altamirano AA,Beaugé L

更新日期：1985-12-01 00:00:00

abstract：:For several years it has been debated whether the Ca-pump in smooth muscle is located in the plasma membrane or in the endoplasmic reticulum (alias sarcoplasmic reticulum). Experimental evidence using skinned smooth muscle cells and subcellular membrane fractions isolated from a number of smooth muscles is reviewed he...

journal_title：Cell calcium

authors： Grover AK

更新日期：1985-06-01 00:00:00

abstract：:The properties of the Ca2+, Mg2+-ATPase of erythrocyte membranes from patients with cystic fibrosis (CF) were extensively compared to that of healthy controls. Following removal of an endogenous membrane inhibitor of the ATPase, activation of the enzyme by Ca2+, calmodulin, limited tryptic digestion or oleic acid, as ...

journal_title：Cell calcium

authors： Waller RL,Johnson LR,Brattin WJ,Dearborn DG

更新日期：1985-06-01 00:00:00

abstract：:C3H/10T1/2 mouse embryo fibroblasts stimulated by a steady electric field (10 V/cm) for 30 min exhibited lamellar retraction on the sides facing the electrodes. Some cells elongated and preferentially oriented with their long axis perpendicular to the field direction. Depletion of external calcium or blockage of calci...

journal_title：Cell calcium

authors： Onuma EK,Hui SW

更新日期：1985-06-01 00:00:00